Manders' Colocalization Coefficients

Calculate Manders' colocalization coefficients between two channels within regions of interest. Returns coefficients M₁ and M₂ or their signal-weighted variants within each region of interest (ROI). Manders' colocalization coefficients are effectively the fraction of signal on one channel that is colocalized with signal on another channel. They are strongly dependent on the selection of appropriate thresholds on each channel, used to define areas of colocalization.

Input Parameters

Image

Expects arbitrary bit-depth images with at least 2 channels.

Type: Image File (net.imagej.Dataset)

Region of Interest

Set of ROIs defining the regions for which correlations will be calculated.

Type: ROI Set (file) (imagej.data.overlay.AbstractOverlay[])

Channel 1 and 2 (1-based index)

First and second channels on which to calculate correlations. Specified using 1-based indeces (1 ≤ x ≤ # of channels).

Type: Integer (int)

Channel 1 and 2 Thresholds

Threshold values for each channel.

Type: Numeric (double)

Signal-weighted

If false, calculate standard Manders' colocalization coefficients. If true, calculate signal-weighted colocalization coefficients.

Type: Logical (boolean)

Results

One result row is produced for each ROI.

M1

Manders' colocalization coefficient for signal on the first channel with signal on the second channel.

Standard:

∑_{A+B}(1) ⁄ ∑_{A}(1)

Signal-weighted:

∑_{A+B}(A_i) ⁄ ∑_{A}(A_i)

A_i, the value on channel 1 at pixel i.
B_i, the value on channel 2 at pixel i.
{A}, the set of pixels where A_i is above the threshold.
{A+B}, the set of pixels where both A_i and B_i are above their respective thresholds.

Type: Numeric (float)

M2

Manders' colocalization coefficient for signal on the second channel with signal on the first channel.